User Tools

Site Tools


transdecoder_for_transcriptomes

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revisionPrevious revision
transdecoder_for_transcriptomes [2017/06/19 06:55] – Formatting 129.173.90.165transdecoder_for_transcriptomes [2017/08/09 13:31] (current) 129.173.94.20
Line 6: Line 6:
    
 1. To run, you will need to make a copy of your transcriptome fasta file in a new folder. Use the command line: 1. To run, you will need to make a copy of your transcriptome fasta file in a new folder. Use the command line:
-  * /opt/perun/TransDecoder-3.0.1/TransDecoder.LongOrfs -t YourTranscriptome.fasta+<code> 
 +/opt/perun/TransDecoder-3.0.1/TransDecoder.LongOrfs -t YourTranscriptome.fasta 
 +</code>
 2. Once this has finished, you will run the TransDecoder Prediction to get the final coding region predictions. Use the following command line, with the same transcriptome file: 2. Once this has finished, you will run the TransDecoder Prediction to get the final coding region predictions. Use the following command line, with the same transcriptome file:
-  * /opt/perun/TransDecoder-3.0.1/TransDecoder.Predict -t YourTranscriptome.fasta +<code> 
 +/opt/perun/TransDecoder-3.0.1/TransDecoder.Predict -t YourTranscriptome.fasta 
 +</code>
 This will result in your final predictions, and the output files that you want to use are the transcoder.pep files.  This will result in your final predictions, and the output files that you want to use are the transcoder.pep files. 
  
 You can rename the headers or remove certain characters you do not want (such as *) by: You can rename the headers or remove certain characters you do not want (such as *) by:
-  * awk '/^>/{print ">" ++i; next}{print}' < inputfile.fasta > outputfile.fasta #This renames the headers to >numbers +<code> 
-  sed 's/*/ /g' inputfilename > outputfilename         #This removes the asterisk characters. +awk '/^>/{print ">" ++i; next}{print}' < inputfile.fasta > outputfile.fasta #This renames the headers to >numbers 
 +sed 's/*/ /g' inputfilename > outputfilename                                #This removes the asterisk characters. 
 +</code>
transdecoder_for_transcriptomes.1497866123.txt.gz · Last modified: by 129.173.90.165