The following is a shell example to run metaspades when short reads are available. #!/bin/bash\\ #$ -S /bin/bash\\ . /etc/profile\\ #$ -cwd\\ #$ -o Blah.log\\ #$ -pe threaded 20\\ cd /to/the/path/containing/the reads/\\ source activate spades\\ metaspades.py -t 20 -m 230 --pe1-1 Blah_R1_PairNtrim.fq --pe1-2 Blah_R2_PairNtrim.fq --pe-s Blah_R1R2unPairNtrim.fq -o /path/to/Metaspades_Blah_output\\ conda deactivate