blast_and_plast
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Here is a shell to run PLAST:
#!/bin/bash #$ -S /bin/bash . /etc/profile #$ -o logo #$ -cwd #$ -pe threaded 10 cd $PWD CPUs=10 DB=/db1/blast-may-2024/nt.nal QF=yourquery.fasta plast -e 1e-10 -max-hit-per-query 1 -outfmt 1 -a $CPUs -p plastn -max-database-size 10000000000 -i $QF -d $DB -o $QF.plout -force-query-order 1000
to parse the output see https://perun.biochem.dal.ca/user-wiki/doku.php?id=taxonomy_recovery
Here is a shell example to run BLAST:
#!/bin/bash #$ -S /bin/bash . /etc/profile #$ -pe threaded 1 #$ -cwd source activate blast export BLASTDB=/db1/blast-may-2024/ DB=nt query=your_query.fasta blastn -db $DB -query $query -out yourqueryresults.blout -num_threads 1 -outfmt "6 qseqid sseqid evalue pident qcovs length slen qlen qstart qend sstart send stitle" source deactivate
Both shells using NCBI nt database (/db1/blast-may-2024/nt.nal), but the formats for specifying DB are different for BLAST and PLAST.
<Last updated by Dandan Zhao on Jun 11, 2024>
blast_and_plast.1718113131.txt.gz · Last modified: by 129.173.94.151
